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完整的透化肌原纖維測試系統

溫度調節組織浴

簡單安裝在倒置顯微鏡上

計算機控制的電動XYZ微定位平臺

包括緩沖閥和解決方案切換器控制器

控制和測量力，長度和肌節間距

測量力-pCa，kTr，剛度，長度 - 張力和力 - 速度關系

與Aurora Scientific 900B高速視頻Sarcomere長度系統（HVSL）無縫連接

參考文獻：

Schappacher-Tilp, Gudrun et al. “Computing Average Passive Forces in Sarcomeres in Length-Ramp Simulations .” PLoS Computational Biology 12.6 (2016): e1004904.

Cornachione, Anabelle S. et al. “The increase in non-cross-bridge forces after stretch of activated striated muscle is related to titin isoforms.” American Journal of Physiology - Cell Physiology 310.1 (2016): C19-C26.

Leite, Felipe S. et al. “Reduced passive force in skeletal muscles lacking protein arginylation.” American Journal of Physiology - Cell Physiology 310.2 (2016): C127-135.

Powers, Krysta et al. “Decreased force enhancement in skeletal muscle sarcomeres with a deletion in titin.” Journal of Experimental Biology 219.9 (2016): 1311-1316.

Minozzo, Fábio C., David Altman, and Dilson E. Rassier. “MgADP activation contributes to force enhancement during fast stretch of isolated skeletal myofibrils.” Biochemical and Biophysical Research Communications 463.4 (2015): 1129-1134.

Philippou, Anastassios et al. “Masticatory muscles of mouse do not undergo atrophy in space.” The FASEB Journal 29.7 (2015): 2769-2779.

Rassier, Dilson, and Aleksander Labuda. “Method and apparatus for measuring cantilever deflection in constrained spaces.” (2014): U.S. Patent No. 8,667,611.

Cornachione, Anabelle S. et al. “Arginylation of Myosin Heavy Chain Regulates Skeletal Muscle Strength.” Cell Reports 8.2 (2014): 470-476.

Yamada, Takashi et al. “Nitrosative modifications of the Ca2+ release complex and actin underlie arthritis-induced muscle weakness.” Annals of the Rheumatic Diseases 74.10 (2014): 1907-1914.

Ribeiro, Paula A.B. et al. “Contractility of myofibrils from the heart and diaphragm muscles measured with atomic force cantilevers: Effects of heart-specific deletion of arginyl-tRNA-protein transferase.” International Journal of Cardiology 168.4 (2013): 3564-3571.

Labuda, Aleksander et al. “Optical detection system for probing cantilever deflections parallel to a sample surface.” Review of Scientific Instruments 82.1 (2011): 013701.

Rassier, Dilson E. “Striated Muscles: From Molecules to Cells.” Advances in Experimental Medicine and Biology Muscle Biophysics (2010): 1-6.

Joumaa, Venus, Walter Herzog, and Dilson E. Rassier. “904-Plat Critical Sarcomere Length Extension and Phase Transition Of Force During Lengthening Of Skeletal Muscle Myofibrils.” Biophysical Journal 94.2 Supplement (2008): 305-307.

1700A肌原纖維系統是第***個商業化的肌原纖維系統，專門用于測量單個肌原纖維激活產生的納米牛頓力。這是***個完整的測試系統，包括表征肌原纖維收縮所需的所有硬件。1700A包括：470A肌原纖維原子力顯微鏡（AFM）傳感器，340A壓電長度控制器，溫控浴槽，470A-XYZ電動平移臺，820A雙XYZ運動控制器，用于計算機控制AFM懸臂和玻璃針定位，溶液閥控制器，解決方案切換電機驅動器和我們的實時Linux 600A數據采集系統。

470 Myofibril AFM傳感器利用原子力顯微鏡和光束偏轉方法測量單個肌原纖維激活產生的納米牛頓力。肌原纖維固定在AFM懸臂和玻璃針之間，AFM懸臂用作高靈敏度力傳感器，玻璃針連接到340A壓電長度控制器。控制長度和測量力使1700A肌原纖維系統能夠提供肌原纖維的完整機械表征。根據所使用的懸臂，滿量程力為1-10μN，驚人的分辨率為1nN。

1700A包含溫度調節組織浴以及緩沖液交換系統的控制，這是快速激活任何肌原纖維的關鍵組分。該設備可以很容易地安裝在倒置顯微鏡上，為浴缸和肌原纖維提供方便和清晰的通道。

用于肌原纖維機械表征的可定制方案可以使用我們的600A數據采集系統進行，當與我們的可選900B / 901C VSL肌節長度測量軟件結合使用時，研究人員可以控制和測量長度，力和肌節長度。